Assessment of Cytotoxicity in Chemotherapeutic Drugs—By Invitro Methods

For past many years, strategies for the preclinical discovery and development of potential anticancer agents have been based largely upon the testing of agents in. selected animals bearing transplantable tumors. The feasibility of implementing combined in vitro/in vivo screening for selective cytotoxicity among panels of human tumor cell lines derived from a broad spectrum of human carcinomas has been evaluated in recent times.. Short-term in vitro drug sensitivity assays have raised the possibility of predicting clinical outcome and selecting optimal components for chemotherapeutic protocols for individual patients. This article discusses various in-vitro methods to access cytotoxicity of different drugs in various cultured malignant tissues.